Clinical Chemistry Quiz No. 5

10 Short answer questions

This Quiz covers the following titles:

  • Important concepts and definitions in clinical chemistry methodologies (Part 2)

1- In fluorescence, the emission light is of longer wavelength than the excitation light (Stokes shift). What is the inner-filter (quenching) effect?

2- Gas chromatography

3- Liquid chromatography (HPLC)

4- What is reverse phase liquid chromatography?

5- What purpose does derivation serve in Gas Chromatography?

6- In reversed phase liquid chromatography, will the more polar HO-C2H5 or the less polar HO-C4H11 elute first?

7- In GC, what can be done to reduce run times? In reversed phase HPLC, what can be done to reduce run times?

8- How are proteins detected in capillary zone electrophoresis? What advantages does capillary electrophoresis have over standard electrophoresis in SPEP?

9- What is a pI? What is an ampholyte? What happens at the pI in isoelectric focusing?

10- For drug assays, what advantages does MS have over immunoassay?