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ECP Atlas
Clinical Chemistry Quiz No. 5
10 Short answer questions
By
Prof. Dr. Hoda Hagras
15-9-2020
This Quiz covers the following titles:
Important concepts and definitions in clinical chemistry methodologies
(Part 2)
1- In fluorescence, the emission light is of longer wavelength than the excitation light (Stokes shift). What is the inner-filter (quenching) effect?
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2- Gas chromatography
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3- Liquid chromatography (HPLC)
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4- What is reverse phase liquid chromatography?
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5- What purpose does derivation serve in Gas Chromatography?
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6- In reversed phase liquid chromatography, will the more polar HO-C2H5 or the less polar HO-C4H11 elute first?
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7- In GC, what can be done to reduce run times? In reversed phase HPLC, what can be done to reduce run times?
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8- How are proteins detected in capillary zone electrophoresis? What advantages does capillary electrophoresis have over standard electrophoresis in SPEP?
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9- What is a pI? What is an ampholyte? What happens at the pI in isoelectric focusing?
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10- For drug assays, what advantages does MS have over immunoassay?
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