8- How are proteins detected in capillary zone electrophoresis? What advantages does capillary electrophoresis have over standard electrophoresis in SPEP?
8- How are proteins detected in capillary zone electrophoresis? What advantages does capillary electrophoresis have over standard electrophoresis in SPEP?
- Proteins detected by optical means, like absorption spectrophotometry and separation is depending on molecular to charge ratio.
- Advantages of capillary electrophoresis:
- Radiates heat readily, does not overheat.
- run times are short because of high voltage
- smaller volume