Question 10 answer

Similar to absorption photometers: high intensity radiation (usually UV range) provided by excitation source (usually mercury vapor, halogen or xenon arc lamp), source light directed through excitation monochromator to isolate excitation wavelength, which goes through sample cuvette. Sample absorbs energy, releases some as photon with different wavelength, which passes through secondary monochromator to isolate emission wavelength, then detected by photomultiplier tube.